The variability of nuclear DNA content of different Pelargonium species estimated by flow cytometry

Pelargonium is a versatile genus mainly from the Cape Region, South Africa. The genus is divided into four subgenera and 16 sections characterized by several groups of chromosomes sizes and numbers. The DNA content of species from all subgenera and sections of Pelargonium, except for the sections Subsucculentia and Campylia was estimated using flow cytometry. Nuclei of Pelargonium samples (leaf or petal tissue) and an internal plant standard (leaf tissue) were isolated together and stained with propidium iodide. The DNA content was estimated providing that the 2C peaks of sample and standard be in linearity in the flow cytometer histograms. In total, 96 Pelargonium accessions of 60 species (22 Pelargonium species for the first time) were analyzed. The 2C DNA content ranged from 0.84 pg (P. longifolium, section Hoarea) to 6.69 pg (P. schizopetalum, section Magnistipulacea) and the corresponding 1Cx DNA content from 0.42 pg (P. longifolium) to 1.72 pg (P. transvaalense. This demonstrates the high plasticity within the genus Pelargonium. Some species, such as P. peltatum accessions revealed a pronounced endopolyploidization in leaves but not in petals underlining the importance to choose the right tissue as sample for the flow cytometry analysis. The reported genome sizes are a step forward towards the characterization of the Pelargonium collection within the German Gene Bank for Ornamental Plants and a valuable base for future sequencing programs of the Pelargonium genomes.


Introduction
Pelargoniums are famous bedding and balcony plants around the world. Pelargonium and Geranium were a common genus until the 18 th century. The remarkable seed shape remembering a cranesbill (crane in Greek: geranos) was the defining feature equal for both. However, the flower architecture is very different: Species of genus Geranium show actinomorphic flowers whereas Pelargonium plants have zygomorphic ones. Thus, Geranium and Pelargonium have been divided into two genera of the family Geraniaceae [1]. Perhaps the long uninterrupted popularity of Pelargonium plants is the reason why the term geranium persists not only in English-speaking countries even in scientific publications. For the sake of correctness, we only use the term Pelargonium.
The genus Pelargonium comprised about 280 taxa [2]. Pelargoniums are mainly distributed in the Cape Region of South Africa [3]. This region is distinguished as a hotspot for its plant diversity and endemism due to very different climate and geographical conditions in a relatively small area. The exceptional climatic stability during the Pleistocene is accepted as a further major factor promoting the abundance of plant species in this region [4]. Species of the genus Pelargonium colonize very different habitats and differ greatly in morphology, anatomy, and cytology. The high number of habits found in Pelargonium probably resulted from the nested radiation in Winter-rainfall region occurred in response to aridification in the mid-Miocene and to the ensuing fragmentation of niches [5][6][7].
Considering the high diversity, the genus is arranged in sixteen infrageneric sections [2,8] of four subgenera [2]. New Pelargonium species are continuously being described [e.g. [9][10][11]. In addition to diverse morphological features, chromosomes of different sizes were found in the respective sections and species [12][13][14][15]. Extensive phylogenetic analyses were performed using various molecular methods [8,[16][17][18]. The remarkable high levels of organelle genomic rearrangements were investigated and phylogenetic analyses on this base confirmed the subgeneric structure of two main clades (small and large chromosome clade) and five subclades [7,19]. Although monophyly has already been demonstrated for some sections as Ligularia and Hoarea, more molecular investigations are necessary to prove this for the other sections as well [7]. In addition, the crown node age for the Pelargonium was dated to 9.7 Mya (Late Miocene) [7]. The accelerated mitochondrial substitution rates and the exceptional variability in the plastome are further outstanding traits of the genus Pelargonium and are the subject to intense research [20][21][22][23][24][25][26][27][28][29].
First pelargoniums came to Europe as early as 1600. Pelargonium species have long been collected in botanical gardens. Nowadays, breeders keep Pelargonium collections as a resource for further crossbreeding to gain a greater genetic variability of the cultivars. Despite a long breeding history, the genetics of commercial cultivars is limited. In Germany, a "subnetwork Pelargonium" was established in the very last years. It belongs to the German Gene Bank for Ornamental plants (DGZ) [30]. The foundation of the DGZ aims to preserve the diversity of ornamental plant genetic resources including Pelargonium and allows the long-term use of these resources. The Pelargonium collection at Julius Kühn Institute (JKI) is a part of it. The collection consists of Pelargonium species and accessions that have been kindly made available by German breeders' houses over the last twenty years. We started to characterize the Pelargonium JKI collection more in detail. Several questions arose regarding the correct botanical classification. To clarify it and to provide more information about the comprehensive Pelargonium JKI collection, we determined the DNA content of the species / accessions using the flow cytometry (FCM).
First, Greilhuber [31] determined a 1C DNA content of 8.1 pg for P. radula by Feulgen cytophotometry. Since the 1980s with the improved equipment of the laboratories, FCM with plant cells developed into a widely used method for determining the plant DNA content [32]. However, DNA amounts in pelargoniums have rarely been determined so far. The values published by different authors differ considerably. For P. x hortorum L.H. Bailey, which traces back to P. zonale and is the most important commercial Pelargonium cultivar group, Cassells et al. [33] stated a 2C DNA content of 3.16 pg while Weng et al. [22] announced a content of 1.79 pg for P. zonale. Nieuwenhuis [34] registered also large differences between his and Weng et al.'s [22] 2C DNA contents for the same Pelargonium species.
In the present paper, we used FCM for the determination of the 2C DNA content in a collection of 60 Pelargonium species and overall 96 accessions. For 22 Pelargonium species, the DNA content was determined for the first time. The investigations should provide information about intraspecific and intrasectional variability of genome size in Pelargonium, help to detect polyploid accessions and support further breeding efforts. With known ploidy the 1Cx content, a valuable feature of the genome, was estimated to prove the hypothesis of genome up-or downsizing in the genus Pelargonium [34,35]. Moreover, experimental challenges of FCM and reasons for very different published DNA contents of Pelargonium species are discussed.

Plant material
Samples for plant DNA flow cytometry were taken from greenhouse plants. The Pelargonium JKI collection consists of 60 Pelargonium species from fourteen sections. More than one accession / subspecies was tested out of 23 species. To maintain healthy young plants, the stock plants are regularly propagated using cuttings. Each accession is represented by at least three plants (Table 1), [S1 Table]. As internal standards a Raphanus sativus L. accession, provided by the Leibniz Institute of Plant Genetics and Crop Plant Research Gatersleben, Germany (Ra) (2C = 1.11 pg) [36] and Solanum lycopersicon L. 'Stupické' (To) (2C = 1.96 pg) [36] were used. Brassica oleracea var. botrytis L. (cauliflower) 'Korso' (Ca) (2C = 1.31 pg) [37] also served as a standard for P. grandiflorum. The standards are kept as in vitro plants on solid medium MS [38] supplemented with 0.2 mg L -1 1-naphthalene acetic acid, 3% sucrose in a climatic chamber (25˚C, 16 hours light / 8 hours dark).

Flow cytometry
Sample plant material was stored in a wet petri dish on ice until preparation according to a modified JKI protocol. Using nuclei extraction and staining buffer of the CyStain 1 PI Absolute P Kit (Sysmex, Germany), propidium iodide (PI) (1 mg/1 mL, Sigma Aldrich), and ribonuclease A (1 mg/1 mL, Serva) samples were prepared. With a sharp razor blade, small pieces of Pelargonium plant material preferably from young leaves, or in few cases also from petals, and leaf pieces from internal standard were chopped up together in 500 μL of nuclei extraction buffer. After adding 1 mL staining solution plus 75 μL PI and 3 μL ribonuclease A, the nuclei suspension was gently shaken and afterwards filtered through a Cell-Strainer Cap (BD Fal-con™) with a pore size of 35 μm. The measurements followed immediately after the sample preparation. At least three separate measurements were performed using the flow cytometer BD FACS Calibur™ (BD Biosciences) or CytoFLEX (Beckman Coulter). The separate measurements were secured by taking material from different plants of an accession or on different days. In cases of ambiguous peaks or a poor quality of the histogram peaks, additional measurements were performed (Table 1). Due to differences in the genome sizes and the occasional occurrence of endopolyploidy, it was sometimes difficult to assign the peaks correctly. In such cases, an overlapping of the peaks from the internal reference standard and Pelargonium could not be excluded. For this reason, measurements without reference standard were carried out followed by comparing the histograms in an overlay design to assign the peaks to corresponding origin. At least 5,000 events were recorded per measurement. For 2C DNA content the mean peak positions of internal reference standard and Pelargonium sample were analyzed supported by the analysis software BD CellQuest Pro (version 5.2.1) or CytExpert 2.3 (Beckman Coulter). The peak quality was assessed according to the CV value (coefficient of variance) and should always be below 5.0. If this was not the case, the measurement was repeated with newly chopped material. The nuclear DNA contents were calculated as proposed by Doležel et al. [39]: Table 1. Pelargonium species / accessions with JKI collection number for which the 2C DNA content was determined in this study. The assignment of sections is based on Röschenbleck et al. [2]. Chromosome numbers, ploidy levels and chromosome lengths were obtained from the literature. 1Cx DNA contents in brackets were calculated with presumable ploidy level (bold). SD: standard deviation, Ra: Raphanus, To: tomato, Ca: cauliflower. Sample 2C value (DNA pg) = Reference 2C value x sample 2C mean peak position /reference 2C mean peak position. The 1Cx DNA content was determined by dividing the 2C DNA content by the known ploidy.

Statistical analysis
Data were analyzed by the statistical software Systat 13 (Germany) using, due to the different samples size of the accessions, the Tukey's b test, α = 5%.

Results
The 2C DNA content of 96 accessions from 60 Pelargonium species is presented (Table 1). Pelargonium is a genus with large differences in genome sizes between the species. The 2C DNA content in the genus ranges between 0.84 pg (P. longifolium) and 6.69 pg (P. schizopetalum) and per sections as follows The quality of the measurements depends on the nuclei isolation procedure, the buffer and mainly on the quality of the plant material. In the present analysis, mostly leaf tissue was used because its easy accessibility. However, in some cases it turned to be difficult to obtain high quality histograms by using leaves as the tissue of choice. In such cases, petals were used as an alternative. As an example, Fig 1 shows representative histograms of nuclei of P. acetosum isolated either from leaf material (Fig 1A) or from petals ( Fig 1B). Moreover, if leaf samples reveal a high degree of endopolyploidization making the interpretation of the histogram peaks difficult, the preparation of stained nuclei from petals can sometimes eliminate these problems. However, since the flowers were not always available, leaves were the most commonly used sample material. In addition to P. acetosum, also for P. cortusifolium, P. peltatum, and P. sidoides satisfying histograms were only obtain with nuclei isolated from petal tissue. In contrast, for P. laxum we could only clearly determine the 2C and the 4C DNA peaks in measurements of leaf tissue. Using petal tissue there was no 2C peak but a conspicuous 4C peak. Measurements with both, leaves and petals on 14 accessions of eight species have shown that the estimated genome size from petal samples was equal or slightly (but significantly) smaller and with one exception (P. vitifolium 39) significantly larger than from leaves samples ( Table 2). The genome sizes were determined using the two internal standards R. sativus or S. lycopersicon. As the only exception, P. grandiflorum was measured with cauliflower 'Korso' ( Table 1). The choice of the standard depended on the position of the 2C sample peak at the x-axis of the histogram and on the CV values. The most important criterion for the selection of the standard was that its 2C DNA peak was close but sufficiently well separate from the 2C DNA Pelargonium peak. For instance, the estimation of the DNA content of P. australe (1.12 pg) and P. echinatum (1.09 pg) was only possible with tomato as internal standard due to an overlap of 2C DNA sample peak with the R. sativus 2C peak. Comparisons of measurements of ten accessions with both standards have shown that in seven accessions the estimated DNA content with tomato is significant lower than that with R. sativus (Fig 2). In three accessions (P. odoratissmum 432, P. acetosum 1/7 and 102) no significant differences between both internal standards were determined, although for P. acetosum 1/7 a higher DNA content was defined with the internal standard tomato.
Due to the breeders interests the Pelargonium JKI collection encompasses especially two sections: the section Pelargonium (25 accessions of 16 species) and Ciconium (26 accessions of 11 species). The 2C DNA content of the diploid species in this section amounted between 0.95 pg (P. grandiflorum) and 1.31 pg (P. tabulare), for the tetraploid ones between 2.13 pg and 2.32 pg, the hexaploid P. capitatum had 3.47 pg and the six 8x accessions ranged between 3.78 pg and 4.49 pg, respectively (Table 1). Within the section Pelargonium, a general relationship

PLOS ONE
between the ploidy level of the accessions and their 1Cx genome size was not found (Fig 3). The lowest 1Cx DNA content was found for the octoploid accession P. graveolens 666 (0.47 pg) and the highest for the diploid P. tabulare 29 (0.66 pg) ( Table 1).
Averaging the 1Cx values over their ploidy level (Table 3), there is an increase from the diploid over the tetraploid species to the hexaploid species, but a significant decrease of mean 1Cx value of the octoploid species compared to the mean 1Cx values of the others ploidy levels ( Table 3).
The section Ciconium includes the species P. zonale and P. peltatum that have so far mostly been used horticulturally. Therefore, this section is of high interest as genetic resource for breeding efforts. We estimated the genome size of 26 accessions of 11 Ciconium species at two ploidy levels, 2x and 4x. For some accessions, the ploidy is not yet clear. Among the diploid accessions, P. peltatum has the smallest genome (2.19 pg) whereas P. tongaense has the largest one with 2.77 pg. At the tetraploid level, a 2C DNA content from 3.57 pg (P. multibracteatum) to 4.55 pg (P. zonale) was determined. Although P. quinquelobatum was described as diploid our measurement of 2C = 4.54 pg indicates that the P. quinquelobatum accession of the JKI collection is tetraploid.
The two tetraploid P. multibracteatum accessions show a noticeable small 1Cx value of 0.89 and 0.90 pg, while 1Cx values of the other accessions of this section are between 1.10 pg (P. peltatum, 2x) and 1.25 pg (P. acraeum, 2x). Pelargonium tongaense (2x) has a strikingly higher 1Cx DNA content of 1.38 pg. Averaging the 1Cx values over the respective ploidy level, the diploid mean 1Cx value (1.15 pg, 20 accessions, 153 samples) is significant higher than tetraploid mean 1Cx value (1.02 pg, 6 accessions, 42 samples). Within the subgenus Paucisignata, which includes the section Ciconium and two unassigned species, one accession of P. caylae revealed the highest 2C DNA content (5.00 pg) and P. transvaalense the highest 1Cx DNA content (1.72 pg), respectively.
Summarizing the 1Cx DNA contents according to the section and ploidy level (Table 4)   and Jenkinsonia. The mean 1Cx DNA content of the genus Pelargonium is 0.89 pg, including unassigned species (Table 1), which are not integrated in Table 4. Independent of the basic chromosome number, sections with a chromosome size < 1.5 μm possess lower 1Cx DNA contents than sections with a chromosome size >1.5 μm. The sections Reniforma (< 1.5 μm; 0.84 pg and 0.86 pg) and Myrrhidum (>1.5 μm; 0.90 pg) mark apparently the transition regarding chromosome and genome size. Considering ploidy levels, 1Cx DNA contents increase until 6x (Hoarea, Pelargonium, Peristera, Reniforma), but decrease at 8x (Pelargonium, Reniforma). As described above, section Ciconium is an exception.
Of the other twelve sections, only two to five species were examined. Despite the small sample size, the high plasticity of the Pelargonium genomes is demonstrated. Especially the representatives of section Reniformia reveal a variable genome size from 1.60 pg for P. ionidiflorum to 6.39 pg for P. sidoides. Obviously, different levels of ploidy exist between and even within the species. In section Magnistipulacea, the 2C DNA content for tetraploid P. bowkeri amounted 2.89 pg and for P. schizopetalum to 6.69 pg. For the latter one, the chromosome number is not yet determined. The three examined accessions of the sections Chorisma and Jenksonia have a similar genome size between 2.37 and 2.91 pg. In section Myrrhidium three varieties of P. myrrhifolium were tested. Two of them have a 2C DNA content of 1.55 and 1.53 pg, respectively. With 2.16 pg P. myrrhifolium var. myrrhifolium differs significantly from the other two varieties.
To investigate the intraspecific variability of 2C DNA content, nine species with three or more accessions were statistically analyzed [S2 Table]. The accessions of the species P. peltatum, P. caylae, and P. fulgidum show no significant intraspecific differences of the 2C DNA content, whereas for the accessions of P. acetosum, P. cucullatum, P. graveolens, P. myrrhifolium, and P. vitifolium a significant intraspecific variability was found. Regarding P. zonale, the Table 4. Summary of the 1Cx DNA contents of the analyzed Pelargonium sections regarding chromosome size, basic chromosome number and ploidy level.

Section
№ Accessions Chromosome size (μm) № of chromo-somes (n) Ploidy level (x) 1Cx DNA content (pg) five diploid accessions do not differ significantly. After chromosome doubling by colchicine treatment the P. zonale accession 509 is tetraploid. This was confirmed again.

Discussion
Species of genus Pelargonium are of interest for both, botanists and ornamental plant producers. In the last century, cytological investigations revealed variability in chromosome number and size [12,14,15,[40][41][42]. With the implementation of molecular methods, Pelargonium phylogenetic relationships were more deeply investigated [5, 7, 19, 22-25, 43, 44]. According to new molecular insights, some changes in the phylogenetic systematics of the genus Pelargonium have been proposed [2,45]. So far, the genome size has been determined for many plants species but information about Pelargonium is limited. In the publicly accessible Plant DNA C-values Database [46] 2C DNA values are only listed for P. radula (16.20 pg) [31] and 28 other Pelargonium species determined by Weng et al. [22]. All of the genome sizes determined by us differ considerably from the values given by Greilhuber [31] and Weng et al. [22], respectively. The largest genome determined by us is that for P. schizopetalum with 6.69 pg (2C). Greilhuber [31] determined the DNA amount by Feulgen method. Pelargonium radula is a synonym for P. radens H.E. Moore [1] belonging to section Pelargonium [12]. We examined the octoploid species P. graveolens and P. vitifolium of the same section and found DNA amounts between 3.78 and 4.49 pg. This was much lower than the DNA amount of 16.20 pg for P. radula found by Greilhuber [31]. Since we observed that the 1Cx content of species belonging to the section Pelargonium varies only a little, the large genome of P. radula is rather surprising. However, since P. radula was not included in our measurements and the chromosome number of the horticultural accession investigated by Greilhuber is given with 2n = 80-82 the reasons for this variation remains elucidated.
With the exception of P. tetragonum, the DNA values determined by Weng et al. [22], which are also published in The Plant DNA C-values Database (Royal Botanic Gardens, Kew), are always much smaller than the DNA values presented here. We can only speculate about the reasons for the substantial deviation from our results. According to Weng et al. [22], the samples were chopped and stained with PI. Arabidopsis thaliana (L.) Heynh. or pre-stained control trout (Oncorhynchus mykiss Walbaum) erythrocytes (DNA control PI #05-7303, Partec 1 , Germany) served as internal standards. The separately stained samples and standards were mixed together immediately before measurement. This type of standardization harbours errors, as the sample and standard were prepared in different environments. The average of the two independent estimates is reported as 2C DNA value. The authors have not given the individual values with each internal standard. Therefore, it is difficult to find the reason for the significant differences from our results. However, we can state at least that both standards are not the best choice. Arabidopsis has a very small DNA amount and often reveals an extensive endopolyploidy [47]. Hence, Arabidopsis is not recommended as reference standard due to a potential misinterpretation of the origin of histogram peaks. The second standard, the prestained control trout erythrocytes, is biologically far away from plant cells that may contain staining inducing substances in its cytosol. Therefore, animal standards are not recommended for plant samples [39,48]. Moreover, Partec 1 , now Sysmex 1 , Germany, the producer of the DNA control PI # 05-7303 advises it as control for the instruments linearity but not for a DNA content determination.
Another comprehensive study on genome size in Pelargonium was published by Nieuwenhuis [34], who collected samples from two botanical gardens. As internal standard Vinca minor L. (2C DNA value = 1.51 pg) was used. Since most of the samples were measured with DAPI, Nieuwenhuis comparatively analysed 14 accessions with DAPI and PI to introduce a conversion factor that allows to correcting the obtained DAPI values for differences in AT-CG base pair portions. Our results are in good concordance with the DNA amounts estimated by Nieuwenhuis [34]. However, large differences were found for few species. Moreover, for many species several cytotypes were reported. From this, we conclude, for example, that our accession P. alchemilloides (4.24 pg) is tetraploid whereas Nieuwenhuis [34] determined a diploid P. alchemilloides (2.15 pg). Some results of the description of Pelargonium JKI collection require further clarification regarding the botanical classification. By handing over the accessions over many years and many hands, errors cannot be ruled out. Furthermore, we cannot exclude breeding efforts such as polyploidization. To our knowledge, this is the first report about a diploid P. crithmifolium (1.37 pg), tetraploid P. quinquelobatum (4.54 pg) and P. oblongatum (1.77 pg) as well as an octoploid P. sidoides (6.39 pg).
Endopolyploidy in plants is a common phenomenon [49]. Few Pelargonium accessions revealed a high degree of endopolyploidy in the leaves. In such cases, it is difficult to avoid misinterpretations of the histogram [50]. Barow and Meister [51] have shown that the degree of endopolyploidization differs between the different organs of a given species and between the different life-cycle types. We repeated measurements at different times throughout the year, have taken leaves at different ages (lower or upper leaves) or, in addition to the leaf material, we also used petals for the measurement. Only in this way, it was possible to determine the 2C peak in P. peltatum, P. acetosum, and P. laxum.
Beside a different histogram quality, DNA contents from petals are lower as DNA contents defined with leaf material or do not differ, with only one exception, namely P. vitifolium. In contrast to our three investigated P. fulgidum accessions, where the DNA content of petal samples was lower, Nieuwenhuis' [34] analysis of leaf and petal samples of one P. fulgidum accession resulted in no significant differences of the DNA content. Furthermore, comparing the applied standards, we have found out that the determined DNA content with internal reference standard R. sativus was equal to or higher than the genome size determined with S. lycopersicon. Therefore, both, the type of tissue and the used internal standard, may influence the DNA content estimation. Greilhuber et al. [48] have already discussed methodological aspects of preparing samples for DNA content measurements with special consideration of standardization and the role of different cytosol compounds as fluorescence inhibitors. Up to now chemical identities of influencing substances from the cytosol are poorly explored [50]. An additional effect could have small particles e.g. coming from trichomes or other parts of the chopped plant tissue. The debris could aggregate with the stained nuclei and can lead to an apparent increase in nuclear fluorescence [52]. Even for a skilled person in the laboratory it is difficult to chop exactly the same amounts from target and standard tissue for preparing the sample. Furthermore, a high amount of extracted secondary metabolites [53] or simply a hidden infestation of the plants with whiteflies [54] could affect the results adversely. Taken these facts altogether it makes standardization between different laboratories difficult or almost impossible and plant 2C DNA contents even for the same species could differ in a small tolerance range. Despite these general drawbacks, flow cytometry is an acknowledged way to determine the genome size of plant species. The advantages over cytological examinations such as simplicity and speed have often been described [32]. Additionally, if the basic chromosome number is known, then the estimation of the 1Cx value is a further valued feature of species. For example, a 1Cx value downsizing was often reported after polyploidization [35,55]. Nieuwenhuis [34] described for the genus Pelargonium a decrease of 1Cx values with increasing ploidy levels during evolution. The extensively examined section Pelargonium with four ploidy levels shows an averaged genome upsizing from the diploid over the tetraploid to the hexaploid species, but a significant genome downsizing of the octoploid species compared to the other ploidy levels. Regarding our results, a general conclusion, if evolutionary or induced polyploidization leads to a genome upsizing or downsizing in the genus Pelargonium, is impossible and further investigations are necessary.
As expected, the chromosome size correlates with the 1Cx value. Species with small chromosomes have a remarkable lower 1Cx value than species with larger chromosomes regardless the basic chromosome number. Interestingly, the three examined accessions of the sections Chorisma and Jenkinsonia, have a similar genome size between 2.37 and 2.91 pg despite similar chromosome size and different basic chromosome number, namely 11 in Chorisma and 9 in Jenkinsonia [15,[56][57][58]. In P. sidoides, P. quinquelobatum, P. oblongatum, and P. crithmifolium the result deviates strongly from the expected 1Cx value. One possible explanation is that the accessions possess a ploidy that has not yet been described. Intraspecific variability in genome size could be explained by the different provenance of the accessions, the existence of subspecies (P. cucullatum [59,60], P. myrrhifolium [61]) or different cytotypes, but also by diverse breeding efforts as induced polyploidization e.g. for P. zonale. Additionally, the here presented and already published 2C and 1Cx DNA contents of the genus Pelargonium [22,34] are summarized in S3 Table. In summary, it could be concluded that plant flow cytometry is a powerful tool for characterization of genetic resources in the genus Pelargonium. For the Pelargonium JKI collection, the flow cytometric data are basics for the plant accession characterization. The data presented here encompass 559 measurements under different conditions with internal standards. Additionally, numerous measurements were performed without internal reference standard for clarifying of the sample 2C and 4C peak position on the histogram. The DNA content was determined for 60 Pelargonium species of it for 22 Pelargonium species for the first time. The reported genome sizes give interesting insights in the accessions of the Pelargonium JKI collection and serve, together with the morphological traits, as a basic passport for the accessions. Furthermore, they are valuable for future Pelargonium genome sequencing programs.
Supporting information S1